Journal: PLoS ONE

Article Title: Stimulated hepatic stellate cell promotes progression of hepatocellular carcinoma due to protein kinase R activation

doi: 10.1371/journal.pone.0212589

Figure Lengend Snippet: (A) Proliferation assay was performed for assessing proliferation of HepG2 cells incubated by conditioning medium from LPS-stimulated or C16-treated LX-2 cells. Mean ± SEM of eight replicates. **P<0.05. (B) Scratch assay was performed for assessing proliferation of HepG2 cells incubated by conditioning medium from LPS-stimulated or C16-treated LX-2 cells. Scale bars represent 500 μm. Mean ± SEM of four replicates. **P<0.05. (C) Invasion assay was performed for assessing invasiveness of HepG2 was performed for assessing proliferation of HepG2 cells incubated by conditioning medium from LPS-stimulated or C16-treated LX-2 cells. Mean ± SEM of four replicates. **P<0.05.

Article Snippet: Cells of the human HCC cell line HepG2 (Japanese Collection of Research Bioresources, Osaka, Japan) were cultured with high glucose DMEM (Thermo Fisher Scientific) supplemented with 10% FBS and 1% penicillin and streptomycin.

Techniques: Proliferation Assay, Incubation, Wound Healing Assay, Invasion Assay

Journal: PLoS ONE

Article Title: Stimulated hepatic stellate cell promotes progression of hepatocellular carcinoma due to protein kinase R activation

doi: 10.1371/journal.pone.0212589

Figure Lengend Snippet: (A) Protein expression of PKR and its phosphorylated form was determined by Western blotting. (B) Following stimulation of the LX-2 cells by palmitic acid, IL-1β mRNA levels were quantified by RT-PCR. Mean ± SEM of four replicates. **P<0.05. (C) The amount of IL-1β in LX-2 cells was measured by ELISA. Mean ± SEM of three replicates. **P<0.05. (D) Proliferation assay was performed for assessing proliferation of HepG2 cells incubated by conditioning medium from palmitic acid-stimulated or C16-treated LX-2 cells. Mean ± SEM of six replicates. **P<0.05. (E) Scratch assay was performed for assessing proliferation of HepG2 cells incubated by conditioning medium from palmitic acid-stimulated or C16-treated LX-2 cells. Scale bars represent 500 μm. Mean ± SEM of four replicates. **P<0.05.

Article Snippet: Cells of the human HCC cell line HepG2 (Japanese Collection of Research Bioresources, Osaka, Japan) were cultured with high glucose DMEM (Thermo Fisher Scientific) supplemented with 10% FBS and 1% penicillin and streptomycin.

Techniques: Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Proliferation Assay, Incubation, Wound Healing Assay